Transgenic mice expressing cre-recombinase specifically in retinal rod bipolar neurons

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Zhang, Xinmei, Chen, Bai-Yu, Hoi-Lam, Alam Ng, Tanner, Julian A, Tay, David, So, Kwok-Fai, Rachel, Rivka A, Copeland, Neal G, Jenkins, Nancy A and Huang, Jian-Dong (2005) Transgenic mice expressing cre-recombinase specifically in retinal rod bipolar neurons. Investigative Ophthalmology & Visual Science, 46 (10). pp. 3515-3520. ISSN 1552-5783


PURPOSE. To establish a transgenic mouse line that expresses Cre-recombinase in retinal rod bipolar cells for the generation of rod bipolar cell-specific knockout mutants. METHODS. The IRES-Cre-cDNA fragment was inserted into a 173-kb bacterial artificial chromosome (BAC) carrying the intact Pcp2 gene, by using red-mediated recombineering. Transgenic mice were generated with the modified BAC and identified. The Cre-transgenic mice were crossed with ROSA26 and Z/EG reporter mice to detect Cre-recombinase activity. RESULTS. X-gal staining showed that strong Cre-recombinase activities were present in retinal inner nuclear layers and cerebellar Purkinje cells. Double staining with an anti-GFP antibody and an anti-PKCα antibody (specific for retinal rod bipolar cells) revealed that Cre-recombinase activity localized exclusively to the rod bipolar cells in the retina. CONCLUSIONS. A mouse BAC-Pcp2-IRES-Cre transgenic line that expresses Cre-recombinase in retinal rod bipolar neurons has been established. Because mutations in some ubiquitously expressed genes may result in retinal degenerative diseases, the mouse strain BAC-Pcp2-IRES-Cre will be a useful new tool for investigating the effects of retinal rod bipolar cell-specific gene inactivation.

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Item type Article
DOI 10.1167/iovs.04-1201
Official URL
Subjects Historical > FOR Classification > 1109 Neurosciences
Historical > FOR Classification > 1113 Optometry and Ophthalmology
Historical > Faculty/School/Research Centre/Department > School of Sport and Exercise Science
Keywords night vision, scotopic conditions, outer plexiform layers, OPLs, rod photoreceptors, form synapses, rod bipolar cells, inner plexiform layers, IPLs, rod pathway, mammalian retina, rod photoreceptors, rod-connected neurons
Citations in Scopus 22 - View on Scopus
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