Selection of High Affinity Ligands to Hepatitis B Core Antigen From a Phage-Displayed Cyclic Peptide Library

Full text for this resource is not available from the Research Repository.

Hoy, K, Yusoff, Khatijah, Seow, Heng-Fong and Tan, Wen Siang (2004) Selection of High Affinity Ligands to Hepatitis B Core Antigen From a Phage-Displayed Cyclic Peptide Library. Journal of Medical Virology , 69 (1). pp. 27-32. ISSN 0146-6615

Abstract

M13 phages that display random disulfide constrained heptapeptides on their gpIII proteins were used to select for high affinity ligands to hepatitis B core antigen (HBcAg). Phages bearing the amino acid sequences C-WSFFSNI-C and C-WPFWGPW-C were isolated, and a binding assay in solution showed that these phages bind tightly to full-length and truncated HBcAg with K D rel values less than 25 nM, which is at least 10 orders of magnitude higher than phage carrying the peptide sequence LLGRMK selected from a linear peptide library. Both the phages that display the constrained peptides were inhibited from binding to HBcAg particles by a monoclonal antibody that binds specifically to the immunodominant region of the particles. A synthetic heptapeptide with the amino acid sequence WSFFSNI derived from one of the fusion peptides inhibits the binding of large surface antigen (L-HBsAg) to core particles with an IC50 value of 12 +/- 2 microM. This study has identified a smaller peptide with a greater inhibitory effect on L-HBsAg-HBcAg association.

Dimensions Badge

Altmetric Badge

Item type Article
URI https://vuir.vu.edu.au/id/eprint/2596
DOI https://doi.org/10.1002/jmv.10266
Official URL http://onlinelibrary.wiley.com/doi/10.1002/jmv.102...
Subjects Historical > Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
Keywords ResPubID19030, biopanning, filamentous phage, dissociation constant, inhibition study, HBV assembly
Citations in Scopus 38 - View on Scopus
Download/View statistics View download statistics for this item

Search Google Scholar

Repository staff login