Isolation and characterization of microsatellite markers in the Southern emu-wren (Stipiturus malachurus: Aves)

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Maguire, G, Guay, Patrick-Jean and Mulder, R (2006) Isolation and characterization of microsatellite markers in the Southern emu-wren (Stipiturus malachurus: Aves). Molecular Ecology Notes, 6 (2). pp. 422-424. ISSN 1471-8278

Abstract

We isolated and characterized eight novel microsatellite loci in the southern emu-wren (Stipiturus malachurus). We used nonradioactive polymerase chain reaction (PCR)-based techniques to screen an enriched genomic DNA library. Based on genotypes from a single population, six loci showed no evidence of null alleles and were polymorphic (allele range = 2–9, mean heterozygosity = 0.57), and one locus was sex-linked (NA = 4). These loci were variable and had different allele size ranges in three other populations of southern emu-wrens, and are therefore useful for determining levels of genetic diversity within and between populations of the species.

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Item type Article
URI https://vuir.vu.edu.au/id/eprint/3033
DOI https://doi.org/10.1111/j.1471-8286.2006.01257.x
Official URL http://onlinelibrary.wiley.com/doi/10.1111/j.1471-...
Subjects Historical > Faculty/School/Research Centre/Department > Institute for Sustainability and Innovation (ISI)
Current > FOR Classification > 0608 Zoology
Current > FOR Classification > 0599 Other Environmental Sciences
Keywords ResPubID17195, Maluridae, microsatellite, population genetics, southern emu-wren, Stipiturus malachurus
Citations in Scopus 9 - View on Scopus
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