Changes in Matrix Metalloproteinase (MMP)-2 and MMP-9 in the Fetal Amnion and Chorion During Gestation and at Term and Preterm Labor

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Yonemoto, H, Young, C. B, Ross, J. T, Guilbert, L. L, Fairclough, R. J and Olson, D. M (2006) Changes in Matrix Metalloproteinase (MMP)-2 and MMP-9 in the Fetal Amnion and Chorion During Gestation and at Term and Preterm Labor. Placenta, 27 (6-7). pp. 669-677. ISSN 0143-4004

Abstract

Increased matrix metalloproteinase (MMP)-9 proteolytic activity is associated with term birth, preterm birth and premature rupture of membranes. However, most studies show no changes with MMP-2, which binds tightly to cell and matrix proteins. We hypothesized better protein extraction would reveal new MMP patterns. Human amnion and chorion were collected from 25 patients at preterm or term, extracted with 2% SDS (a high concentration), and the MMP protein levels and pro-enzyme activities were determined by Western immunoblotting and zymography. MMP-2 protein and MMP-2 and -9 pro-enzyme activities in the amnion increased significantly (p<0.05) with labor at term, and were higher than at preterm labor (p<0.05), when extracted with high SDS concentration. There were no changes in chorion MMPs under any condition. These associations suggest MMP-2 may be another regulator of membrane rupture and other labor-associated mechanisms at term parturition, and its role(s) should be examined further.

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Item type Article
URI https://vuir.vu.edu.au/id/eprint/3133
DOI https://doi.org/10.1016/j.placenta.2005.05.014
Official URL http://dx.doi.org/10.1016/j.placenta.2005.05.014
Subjects Historical > FOR Classification > 1114 Paediatrics and Reproductive Medicine
Historical > FOR Classification > 1101 Medical Biochemistry and Metabolomics
Historical > Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
Keywords ResPubID19281, matrix metalloproteinases, MMP-2; MMP-9, amnion, chorion, gestation, preterm labor, term labor
Citations in Scopus 52 - View on Scopus
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