Immunolocalization of ACE2 and AT2 Receptors in Rabbit Atherosclerotic Plaques

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Zulli, Anthony, Burrell, Louise M, Widdop, Robert E, Black, M. Jane, Buxton, Brian F and Hare, David L (2006) Immunolocalization of ACE2 and AT2 Receptors in Rabbit Atherosclerotic Plaques. Journal of Histochemistry and Cytochemistry, 54 (2). pp. 147-150. ISSN 0022-1554


Evidence suggests that angiotensin type 2 receptor (AT2R) and angiotensin-converting enzyme 2 (ACE2) play a protective role in atherogenesis. These factors have not been identified in rabbit atherosclerotic plaques. Our goal was to localize ACE2 and AT2R in rabbit atherosclerotic tissues, and determine which cell types express these factors. New Zealand White rabbits were fed either a control diet or a 0.5% cholesterol diet (n=8/group) for 12 weeks. Paraffin-fixed thoracic aorta were serially sectioned and processed for immunohistochemistry using commercially available antibodies to ACE2, AT2R, RAM 11 (to identify macrophages), and α smooth muscle cell actin (αSMC) to identify smooth muscle cells and myofibroblasts. AT2R immunoreactivity, but not ACE2 immunoreactivity, was clearly present in endothelia overlying normal wall. However, both AT2R and ACE2 immunoreactivity were clearly present in endothelia overlying neo-intima formation and atherosclerotic plaques. Within plaques, both AT2R and ACE2 immunoreactivity were observed in macrophages and αSMC actin-positive cells. Examination of serial sections showed that the majority of cells were both ACE2- and AT2R-positive. Macrophages and αSMC actin-positive cells produce ACE2 and the AT2R in atherosclerotic plaques. Determining a role for these factors in the control of atherosclerosis will require additional studies

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Item type Article
DOI 10.1369/jhc.5C6782.2005
Official URL
Subjects Historical > FOR Classification > 1102 Cardiorespiratory Medicine and Haematology
Historical > FOR Classification > 1101 Medical Biochemistry and Metabolomics
Historical > Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
Keywords ResPubID17588, atherosclerosis, ACE2, AT2 receptors, immunohistochemistry
Citations in Scopus 59 - View on Scopus
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