Isolation and characterization of polymorphic microsatellite loci for the study of paternity and population structure in the little penguin Eudyptula minor

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Billing, T. M, Guay, Patrick-Jean, Peucker, A. J and Mulder, R. A (2007) Isolation and characterization of polymorphic microsatellite loci for the study of paternity and population structure in the little penguin Eudyptula minor. Molecular Ecology Notes, 7. pp. 425-427. ISSN 1471-8278

Abstract

We isolated and characterized eight novel microsatellite loci in the little penguin Eudyptula minor, using nonradioactive polymerase chain reaction-based techniques to screen GA and GAAA repeats from enriched genomic DNA libraries. All eight loci were polymorphic and seven were variable in our main study population. Cross-amplification using a microsatellite primer developed in Spheniscus demersus(African penguin) yielded one additional polymorphic locus. This locus combined with six of the little penguin loci is suitable for paternity assignment in little penguins.

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Item type Article
URI https://vuir.vu.edu.au/id/eprint/3189
DOI https://doi.org/10.1111/j.1471-8286.2006.01607.x
Official URL http://onlinelibrary.wiley.com/doi/10.1111/j.1471-...
Subjects Historical > Faculty/School/Research Centre/Department > Institute for Sustainability and Innovation (ISI)
Current > FOR Classification > 0608 Zoology
Keywords ResPubID17193, Eudyptula minor, little penguin, microsatellite, parentage, paternity, population genetics
Citations in Scopus 11 - View on Scopus
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