Quantitative polarized phase microscopy for birefringence imaging

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Dragomir, Nicoleta, Goh, Xiao Ming, Curl, Claire L, Delbridge, Lea M. D and Roberts, Ann (2007) Quantitative polarized phase microscopy for birefringence imaging. Optics Express, 15 (26). pp. 17690-17698. ISSN 1094-4087

Abstract

A novel application of quantitative phase imaging under linearly polarized light is introduced for studying unstained anisotropic live cells. The method is first validated as a technique for mapping the two-dimensional retardation distribution of a well-characterized optical fiber and is then applied to the characterization of unstained isolated cardiac cells. The experimental retardation measurements are in very good agreement with the established Brace-K hler method, and additionally provide spatially resolved cell birefringence and phase data.

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Item type Article
URI https://vuir.vu.edu.au/id/eprint/3247
DOI https://doi.org/10.1364/OE.15.017690
Official URL http://www.opticsinfobase.org/oe/abstract.cfm?uri=...
Subjects Historical > Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
Current > FOR Classification > 0205 Optical Physics
Keywords ResPubID16372, ResPubID18563. quantitative phase imaging, mapping two-dimensional retardation, optical fibers, cardiac cells, Brace-K hler method, cell birefringence
Citations in Scopus 23 - View on Scopus
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