Feehan, Jack ORCID: 0000-0002-9627-1299, Nurgali, Kulmira ORCID: 0000-0002-2597-6929, Apostolopoulos, Vasso ORCID: 0000-0001-6788-2771 and Duque, Gustavo ORCID: 0000-0001-8126-0637 (2021) Development and validation of a new method to isolate, expand, and differentiate circulating osteogenic precursor (COP) cells. Bone Reports, 15. ISSN 2352-1872

Abstract

Circulating osteogenic precursor (COP) cells are a population of progenitor cells in the peripheral blood with the capacity to form bone in vitro and in vivo. They have characteristics of the mesenchymal stem and progenitor pool found in the bone marrow; however, more recently, a population of COP cells has been identified with markers of the hematopoietic lineage such as CD45 and CD34. While this population has been associated with several bone pathologies, a lack of cell culture models and inconsistent characterization has limited mechanistic research into their behavior and physiology. In this study, we describe a method for the isolation of CD45+/CD34+/alkaline phosphatase (ALP) + COP cells via fluorescence-activated cell sorting, as well as their expansion and differentiation in culture. Hematopoietic COP cells are a discreet population within the monocyte fraction of the peripheral blood mononuclear cells, which form proliferative, fibroblastoid colonies in culture. Their expression of hematopoietic markers decreases with time in culture, but they express markers of osteogenesis and deposit calcium with differentiation. It is hoped that this will provide a standard for their isolation, for consistency in future research efforts, to allow for the translation of COP cells into clinical settings.

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