A rigorous method to enrich for exosomes from brain tissue

Vella, Laura J ORCID: 0000-0002-1869-6291, Scicluna, Benjamin J ORCID: 0000-0001-6828-9910, Cheng, Lesley ORCID: 0000-0002-8075-6144, Bawden, Emma G, Masters, Colin L ORCID: 0000-0003-3072-7940, Ang, Ching-Seng, Willamson, Nicholas, McLean, Catirona ORCID: 0000-0002-9259-8411, Barnham, Kevin J ORCID: 0000-0003-1164-9465 and Hill, Andrew F ORCID: 0000-0001-5581-2354 (2017) A rigorous method to enrich for exosomes from brain tissue. Journal of Extracellular Vesicles, 6 (1). ISSN 2001-3078


Extracellular vesicles, including exosomes, are released by all cells, including those of the nervous system. Capable of delivering lipid, protein and nucleic acids to both nearby and distal cells, exosomes have been hypothesized to play a role in progression of many diseases of the nervous system. To date, most analyses on the role of these vesicles in the healthy and diseased state have relied on studying vesicles from in vitro sources, such as conditioned cell culture media, or body fluids. Here we have taken a critical approach to the enrichment and characterization of exosomes from human frontal cortex. This method maintains the integrity of the vesicles and their cargo, and comprehensive proteomic and genomic characterization confirms the legitimacy of the resulting extracellular vesicles as endosome-derived exosomes. This method will enable neuroscientists to acquire more detailed information about exosomes in the brain and explore the role(s) this form of intercellular communication and unique source of lipid, protein and RNA has in healthy brain function and pathogenic conditions. Furthermore, this method may have important utility in the isolation of exosomes from other tissues.

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Item type Article
URI https://vuir.vu.edu.au/id/eprint/45760
DOI 10.1080/20013078.2017.1348885
Official URL https://www.tandfonline.com/doi/full/10.1080/20013...
Subjects Current > FOR (2020) Classification > 3101 Biochemistry and cell biology
Current > Division/Research > Chancellery
Keywords extracellular vesicles, EVs, brain health, brain tissue, exomes
Citations in Scopus 146 - View on Scopus
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