Ubiquitin-like protein 3 (UBL3) is required for MARCH ubiquitination of major histocompatibility complex class II and CD86

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Liu, Haiyin ORCID: 0000-0003-3103-6949, Wilson, Kayla R ORCID: 0000-0003-2023-7964, Firth, Ashley M ORCID: 0000-0002-2076-1363, Macri, Christophe, Schriek, Patrick, Blum, Annabelle ORCID: 0000-0002-0034-3527, Villar, Javiera ORCID: 0000-0002-8581-4155, Wormald, Samuel, Shambrook, Mitch, Xu, Bangyan ORCID: 0000-0002-2156-9074, Lim, Hui Jing ORCID: 0000-0001-5045-4391, McWilliam, Hamish EG ORCID: 0000-0003-3479-3419, Hill, Andrew F ORCID: 0000-0001-5581-2354, Edgington-Mitchell, Laura E, Caminschi, Irinia, Lahoud, Mireille ORCID: 0000-0001-8472-6201, Segura, Elodie ORCID: 0000-0003-1795-1921, Herold, Marco J ORCID: 0000-0001-7539-7581, Villadangos, Jose A ORCID: 0000-0001-6771-8891 and Mintern, Justine D ORCID: 0000-0002-3797-8577 (2022) Ubiquitin-like protein 3 (UBL3) is required for MARCH ubiquitination of major histocompatibility complex class II and CD86. Nature Communications, 13. ISSN 2041-1723


The MARCH E3 ubiquitin (Ub) ligase MARCH1 regulates trafficking of major histocompatibility complex class II (MHC II) and CD86, molecules of critical importance to immunity. Here we show, using a genome-wide CRISPR knockout screen, that ubiquitin-like protein 3 (UBL3) is a necessary component of ubiquitination-mediated trafficking of these molecules in mice and in humans. Ubl3-deficient mice have elevated MHC II and CD86 expression on the surface of professional and atypical antigen presenting cells. UBL3 also regulates MHC II and CD86 in human dendritic cells (DCs) and macrophages. UBL3 impacts ubiquitination of MARCH1 substrates, a mechanism that requires UBL3 plasma membrane anchoring via prenylation. Loss of UBL3 alters adaptive immunity with impaired development of thymic regulatory T cells, loss of conventional type 1 DCs, increased number of trogocytic marginal zone B cells, and defective in vivo MHC II and MHC I antigen presentation. In summary, we identify UBL3 as a conserved, critical factor in MARCH1-mediated ubiquitination with important roles in immune responses.

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Item type Article
URI https://vuir.vu.edu.au/id/eprint/46651
DOI 10.1038/s41467-022-29524-w
Official URL https://www.nature.com/articles/s41467-022-29524-w
Subjects Current > FOR (2020) Classification > 3404 Medicinal and biomolecular chemistry
Current > Division/Research > Institute for Health and Sport
Keywords trafficking, proteins, ubiquination, cell surface abundance
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