AL-Attabi, Riyadh, Merenda, Andrea, Hsia, Tina, Sriramoju, Bhasker, Dumée, Ludovic F, Thang, San H, Pham, Hung, Yang, Xing ORCID: 0000-0002-8403-8254 and Kong, Lingxue ORCID: 0000-0001-6219-3897 (2024) Morphology engineering of nanofibrous poly(acrylonitrile)-based strong anion exchange membranes for enhanced protein adsorption and recovery. Journal of Water Process Engineering, 65. p. 105750. ISSN 2214-7144

Abstract

The (bio)pharmaceutical and food industries are transitioning towards circular economy to minimise waste and foster sustainability in the supply chain of biomolecule-based drugs and supplements. Membrane chromatography is expected to play a crucial role in this transition, with the potential to maximize recovery and reduce operating costs. The development of chromatographical membranes is however impeded by the lack of understanding on the membrane structure – chemistry – performance relationship. Here a one-pot synthesis of anion exchange membranes with engineered nanofibrous morphology was studied by electrospinning the blend of polyacrylonitrile (PAN) and tailored quaternized poly(acrylonitrile)-co-poly(2-(dimethylamino)ethyl acrylate) copolymer containing 15 mol% of functional groups, namely PAN-pAQ membranes. Comprehensive evaluations were conducted on the influence of the electrospinning parameters on the nanofibre morphology, surface properties and binding capacity of model protein bovine serum albumin (BSA). The fibre alignment was shown to play a significant role, i.e., membrane with anisotropic fibres obtained by increasing the drum rotational speed to 2000 rpm allowed a high BSA binding capacity of 166 mg∙g−1, i.e., 45–66 % higher than other membranes with randomly-oriented nanofibres. This study is expected to contribute towards the design of future membranes for effective purification and recovery of biomolecules from natural and waste streams, promoting circular bio-economy.

Search Google Scholar

Repository staff login