Gas-phase formation and reactions of radical cations of guanosine, deoxyguanosine and their homodimers and heterodimers

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Feketeova, Linda, Yuriev, Elizabeth, Orbell, John ORCID: 0000-0003-3485-5309, Khairallah, George N and O'Hair, Richard A. J (2011) Gas-phase formation and reactions of radical cations of guanosine, deoxyguanosine and their homodimers and heterodimers. International Journal of Mass Spectrometry, 304 (2-3). pp. 74-82. ISSN 1387-3806


Electrospray ionisation of methanolic solutions containing a mixture of the nucleoside deoxyguanosine, dG, incubated with Cu(NO3)2 resulted in the formation of a range of ions, including doubly charged copper nucleoside complexes [CuIIdGn]2+, with n ranging from 2 to 10. Collision-induced dissociation of these complexes proceeds via a number of different pathways that depend on the size of the cluster, n. When n = 3, monomeric radical cations are formed via redox processes. When n = 4, dimeric radical cations are formed. Related complexes are formed for the nucleoside guanosine, Gs, and these [CuIIGsn]2+ complexes fragment in similar fashions to their [CuIIdGn]2+ counterparts. A key finding is that the radical cations of dG and Gs have fragmentation patterns that depend on the way they are formed. Thus radical cations, dG•+ and Gs•+, formed directly in the electrospray ionisation source or via collision-induced dissociation of [CuIIdG3]2+ and [CuIIGs3]2+ complexes fragment in the same way, giving the radical cation of the guanine base at m/z 151 via cleavage of the N-glycosidic bond. In contrast, the collision-induced dissociation spectra of radical cations formed via the sequences [CuIIdG4]2+→ dG2 •+→ dG•+ and [CuIIGs4]2+→ Gs2 •+→ Gs•+ are dominated by the loss of CH2O and further loss of C2H3O2 from the sugar moiety. These different fragmentation reactions are attributed to different tautomeric structures of the radical cations. Quantum chemical calculations were carried out on possible structures of the radical cation dimer of the model 9-methylguanine. Three low energy structures were found. Two of these represent base pairs of the kind found in supramolecular motifs of guanine derivatives, and one of these possesses a novel tautomeric structure that may have important biological implications.

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Special edition: Nucleic Acids

Item type Article
DOI 10.1016/j.ijms.2010.04.012
Official URL
Subjects Historical > FOR Classification > 0202 Atomic, Molecular, Nuclear, Particle and Plasma Physics
Historical > FOR Classification > 1004 Medical Biotechnology
Historical > Faculty/School/Research Centre/Department > School of Engineering and Science
Keywords ResPubID21618, ResPubID23059, radical cations, deoxyguanosine, guanosine, Copper(II) complexes, tautomers
Citations in Scopus 18 - View on Scopus
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