Effects of Age and Sedentary Lifestyle on Skeletal Muscle NF-κB Signaling in Men

Full text for this resource is not available from the Research Repository.

Buford, Thomas W, Cooke, Matthew, Manini, T, Leeuwenburgh, C and Willoughby, D (2010) Effects of Age and Sedentary Lifestyle on Skeletal Muscle NF-κB Signaling in Men. Journals of Gerontology. Series A: Biological Sciences & Medical Sciences , 65A (5). pp. 532-537. ISSN 1079-5006


Nuclear factor kappa B (NF-κB) is a critical signaling molecule of disuse-induced skeletal muscle atrophy. However, few studies have carefully investigated whether similar pathways are modulated with physical activity and age. The present study examined lean mass, maximal force production, and skeletal muscle NF-κB signaling in 41 men categorized as sedentary (OS, N = 13, 63.85 ± 6.59 year), physically active (OA, N = 14, 60.71 ± 5.54 year), or young and sedentary (YS, N = 14, 21.35 ± 3.84 year). Muscle tissue from the vastus lateralis was assayed for messenger RNA (mRNA) expression of the β subunit of IkB kinase (IKKβ), cytosolic protein content of phosphorylated inhibitor of kappa B alpha (pIKBα), and nuclear content of NF-κB subunits p50 and p65. When compared with YS, OS demonstrated age-related muscle atrophy and reduced isokinetic knee extension torque. Physical activity in older individuals preserved maximal isokinetic knee extension torque. OS muscle contained 50% more pIKBα than OA and 61% more pIKBα than YS. Furthermore, nuclear p65 was significantly elevated in OS compared with YS. OS muscle did not differ from either of the other two groups for nuclear p50 or for mRNA expression of IKKβ. These results indicate that skeletal muscle content of nuclear-bound p65 is elevated by age in humans. The elevation in nuclear-bound p65 appears to be at least partially due to significant increases in pIKBα. A sedentary lifestyle appears to play some role in increased IKBα; however, further research is needed to identify downstream effects of this increase.

Dimensions Badge

Altmetric Badge

Additional Information

Online ISSN: 1758-535X

Item type Article
URI https://vuir.vu.edu.au/id/eprint/7540
DOI 10.1093/gerona/glp196
Official URL http://biomedgerontology.oxfordjournals.org/conten...
Subjects Historical > Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
Historical > FOR Classification > 1101 Medical Biochemistry and Metabolomics
Historical > FOR Classification > 1106 Human Movement and Sports Science
Keywords ResPubID21803. sarcopenia, muscle atrophy, muscle strength, proteolysis, age, sedentary lifestyle, skeletal muscle, nuclear-bound p65, NF-κB, nuclear factor kappa B, IKBα
Citations in Scopus 68 - View on Scopus
Download/View statistics View download statistics for this item

Search Google Scholar

Repository staff login