Buford, Thomas W, Cooke, Matthew and Willoughby, Darryn (2009) Resistance exercise-induced changes of inflammatory gene expression within human skeletal muscle. European Journal of Applied Physiology, 107 (4). pp. 463-471. ISSN 1439-6319

Abstract

Aberrant local inXammatory signaling within skeletal muscle is now considered a contributing factor to the development of sarcopenia. Recent evidence indicates that chronic resistance training contributes to the control of locally derived inXammation via adaptations to repeated, acute increases in pro-inXammatory mRNA within muscle. However, only a limited number of gene transcripts related to the inXammatory process have been examined in the literature. The present study utilized an acute bout to examine the eVects of resistance exercise on several inXammatoryrelated genes in 24 physically active, post-menopausal women not currently undergoing hormone replacement therapy. Following a standard warm-up, participants completed a lower-body resistance exercise bout consisting of 3 sets of 10 repetitions on machine squat, leg press, and leg extension exercises (80% intensity). Muscle biopsies were obtained from the vastus lateralis of the dominant leg at baseline and 3 h following exercise. SigniWcant (p < 0.05) up-regulation in mRNA content was observed for TNF-alpha, IL1-beta, IL6, IL8, SOCS2, COX2, SAA1, SAA2, IKKB, cfos, and junB. Muscle mRNA content was not signiWcantly altered at the 0.05 level for IL2, IL5, IL10, or IL12 (p35). Venous blood samples were also obtained at baseline as well as at 3, 24, and 48 h post-exercise. Serum was analyzed for circulating TNF-alpha, IL1-beta, IL6, IL8, COX2, and SAA with no signiWcant changes observed. These results indicate that resistance exercise is capable of up-regulating transcription of numerous inXammatory mediators within skeletal muscle, and these appear to be worthy of future examination in chronic studies.

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