Nucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptake-encoding region

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Blatch, Gregory ORCID: 0000-0003-0778-8577, Scholle, R and Woods, David R (1990) Nucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptake-encoding region. Gene, 95 (1). pp. 17-23. ISSN 0378-1119

Abstract

The nucleotide sequence of the Vibrio alginolyticus sucrose uptake-encoding region was determined, and contained two genes, scrA and scrK. The scrA gene encodes an enzyme IISucrose (EIIScr) protein of the phosphoenolpyruvate dependent phosphotransferase system and the scrK gene encodes a fructokinase. The deduced amino acid (aa)_sequence for the V. alginolyticus EIIScr protein was homologous with the EIIScr protein from Streptococcus mutants, Salmonella typhimurium (pUR400 system) and Bacillus subtilis. The deduced aa sequence for the V. alginolyticus fructokinase was homologous with the Escherichia coli enzymes, 6-phosphofructokinase (isoenzyme 2) and ribokinase. Transposon phoA mutagenesis experiments indicated that the EIIScr protein was a membrane-bound protein with a region that extended into the periplasm.

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Item type	Article
URI	https://vuir.vu.edu.au/id/eprint/7731
DOI	10.1016/0378-1119(90)90408-J
Official URL	http://dx.doi.org/10.1016/0378-1119(90)90408-J
Subjects	Historical > Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences Historical > FOR Classification > 0604 Genetics
Keywords	ResPubID22242. Bacillus subtilis, Streptococcus mutants, sucrase, fructokinase, transposon, recombinant DNA
Citations in Scopus	29 - View on Scopus
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