GeneClip™ U1 Hairpin Cloning Systems for expression of short hairpin RNAs in vivo
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Yeager, Thomas, Bailey, Cheryl, Vidugiriene, J, Almond, B and Storts, D (2005) GeneClip™ U1 Hairpin Cloning Systems for expression of short hairpin RNAs in vivo. Promega Notes, 89 (1). pp. 21-24. ISSN n/a
Abstract
The GeneClip™ U1 Hairpin Cloning Systems enable cloning and expression of short hairpin RNAs (shRNAs) for RNA interference (RNAi) experiments. The GeneClip™ Systems contain linearized vectors that facilitate cloning of complementary oligonucleotides downstream of a U1 promoter. Upon transfection with pGeneClip™ Vectors containing these cloned sequences, shRNA is produced in the cell, leading to reduction of target gene expression through RNAi. In this article we illustrate use of the GeneClip™ Systems to suppress Renilla luciferase expression in a range of cell types and to suppress p53 expression in both a transient and stable manner.
| Item type | Article |
| URI | https://vuir.vu.edu.au/id/eprint/7824 |
| Official URL | http://www.promega.com/resources/articles/pubhub/p... |
| Subjects | Historical > FOR Classification > 0604 Genetics Historical > Faculty/School/Research Centre/Department > School of Engineering and Science |
| Keywords | ResPubID22570. hairpin RNAs, GeneClip™ Hairpin Cloning Systems, RNA, genes |
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