AMPK activation is fiber type specific in human skeletal muscle: effects of exercise and short-term exercise training

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Lee-Young, Robert S, Canny, Benedict J, Myers, Damian E and McConell, Glenn ORCID: 0000-0002-8572-9065 (2009) AMPK activation is fiber type specific in human skeletal muscle: effects of exercise and short-term exercise training. Journal of Applied Physiology, 107 (1). pp. 283-289. ISSN 8750-7587


AMP-activated protein kinase (AMPK) has been extensively studied in whole muscle biopsy samples of humans, yet the fiber type-specific expression and/or activation of AMPK is unknown. We examined basal and exercise AMPK-α Thr172 phosphorylation and AMPK subunit expression (α1, α2, and γ3) in type I, IIa, and IIx fibers of human skeletal muscle before and after 10 days of exercise training. Before training basal AMPK phosphorylation was greatest in type IIa fibers (P < 0.05 vs. type I and IIx), while an acute bout of exercise increased AMPK phosphorylation in all fibers (P < 0.05), with the greatest increase occurring in type IIx fibers. Exercise training significantly increased basal AMPK phosphorylation in all fibers, and the exercise-induced increases were uniformly suppressed compared with pretraining exercise. Expression of AMPK-α1 and -α2 was similar between fibers and was not altered by exercise training. However, AMPK-γ3 was differentially expressed in skeletal muscle fibers (type IIx > type IIa > type I), irrespective of training status. Thus skeletal muscle AMPK phosphorylation and AMPK expression are fiber type specific in humans in the basal state, as well as during exercise. Our findings reveal fiber type-specific differences that have been masked in previous studies examining mixed muscle samples.

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Item type Article
DOI 10.​1152/​japplphysiol.​91208.​2008
Official URL
Subjects Historical > FOR Classification > 1106 Human Movement and Sports Science
Historical > Faculty/School/Research Centre/Department > Institute of Sport, Exercise and Active Living (ISEAL)
Keywords ResPubID21992. muscle fibres, muscles, phosphorylation, immunohistochemistry, contraction
Citations in Scopus 60 - View on Scopus
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