Skeletal muscle neuronal nitric oxide synthase μ protein is reduced in people with impaired glucose homeostasis and is not normalized by exercise training

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Bradley, Scott J, Kingwell, Bronwyn A, Canny, Benedict J and McConell, Glenn ORCID: 0000-0002-8572-9065 (2007) Skeletal muscle neuronal nitric oxide synthase μ protein is reduced in people with impaired glucose homeostasis and is not normalized by exercise training. Metabolism: Clinical and Experimental, 56 (10). pp. 1405-1411. ISSN 0026-0495

Abstract

Skeletal muscle inducible nitric oxide synthase (NOS) protein is greatly elevated in people with type 2 diabetes mellitus, whereas endothelial NOS is at normal levels. Diabetic rat studies suggest that skeletal muscle neuronal NOS (nNOS) μ protein expression may be reduced in human insulin resistance. The aim of this study was to determine whether skeletal muscle nNOSμ protein expression is reduced in people with impaired glucose homeostasis and whether exercise training increases nNOSμ protein expression in these individuals because exercise training increases skeletal muscle nNOSμ protein in rats. Seven people with type 2 diabetes mellitus or prediabetes (impaired fasting glucose and/or impaired glucose tolerance) and 7 matched (sex, age, fitness, body mass index, blood pressure, lipid profile) healthy controls aged 36 to 60 years participated in this study. Vastus lateralis muscle biopsies for nNOSμ protein determination were obtained, aerobic fitness was measured (peak pulmonary oxygen uptake [Vo2 peak]), and glucose tolerance and insulin homeostasis were assessed before and after 1 and 4 weeks of cycling exercise training (60% Vo2 peak, 50 minutes × 5 d wk−1). Skeletal muscle nNOSμ protein was significantly lower (by 32%) in subjects with type 2 diabetes mellitus or prediabetes compared with that in controls before training (17.7 ± 1.2 vs 26.2 ± 3.4 arbitrary units, P < .05). The Vo2 peak and indicators of insulin sensitivity improved with exercise training in both groups (P < .05), but there was no effect of exercise training on skeletal muscle nNOSμ protein in either group. In conclusion, individuals with impaired glucose homeostasis have reduced skeletal muscle nNOSμ protein content. However, because exercise training improves insulin sensitivity without influencing skeletal muscle nNOSμ protein expression, it seems that changes in skeletal muscle nNOSμ protein are not central to the control of insulin sensitivity in humans and therefore may be a consequence rather than a cause of diabetes.

Item type Article
URI https://vuir.vu.edu.au/id/eprint/8027
Official URL http://dx.doi.org/10.1016/j.metabol.2007.06.003
Subjects Historical > FOR Classification > 1101 Medical Biochemistry and Metabolomics
Historical > FOR Classification > 1106 Human Movement and Sports Science
Historical > Faculty/School/Research Centre/Department > Institute of Sport, Exercise and Active Living (ISEAL)
Keywords ResPubID22068. skeletal muscle, nitric oxide synthase protein, glucose homeostasis, exercise, NOS, insulin, diabetes
Citations in Scopus 27 - View on Scopus
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