High-intensity exercise acutely decreases the membrane content of MCT1 and MCT4 and buffer capacity in human skeletal muscle

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Bishop, David, Edge, Johann, Thomas, Claire and Mercier, Jacques (2007) High-intensity exercise acutely decreases the membrane content of MCT1 and MCT4 and buffer capacity in human skeletal muscle. Journal of Applied Physiology, 102 (2). pp. 616-621. ISSN 8750-7587

Abstract

The regulation of intracellular pH during intense muscle contractions occurs via a number of different transport systems [e.g., monocarboxylate transporters (MCTs)] and via intracellular buffering (betamin vitro). The aim of this study was to investigate the effects of an acute bout of high-intensity exercise on both MCT relative abundance and betamin vitro in humans. Six active women volunteered for this study. Biopsies of the vastus lateralis were obtained at rest and immediately after 45 s of exercise at 200% of maximum O2 uptake. betamin vitro was determined by titration, and MCT relative abundance was determined in membrane preparations by Western blots. High-intensity exercise was associated with a significant decrease in both MCT1 (–24%) and MCT4 (–26%) and a decrease in betamin vitro (–11%; 135 ± 3 to 120 ± 2 µmol H+·g dry muscle–1·pH–1; P < 0.05). These changes were consistently observed in all subjects, and there was a significant correlation between changes in MCT1 and MCT4 relative abundance (R2 = 0.92; P < 0.05). In conclusion, a single bout of high-intensity exercise decreased both MCT relative abundance in membrane preparations and betamin vitro. Until the time course of these changes has been established, researchers should consider the possibility that observed training-induced changes in MCT and betamin vitro may be influenced by the acute effects of the last exercise bout, if the biopsy is taken soon after the completion of the training program. The implications that these findings have for lactate (and H+) transport following acute, exhaustive exercise warrant further investigation.

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Additional Information

Online ISSN: 1522-1601

Item type Article
URI https://vuir.vu.edu.au/id/eprint/8052
DOI https://doi.org/10.1152/japplphysiol.00590.2006
Official URL http://dx.doi.org/10.1152/japplphysiol.00590.2006
Subjects Historical > Faculty/School/Research Centre/Department > Institute of Sport, Exercise and Active Living (ISEAL)
Current > FOR Classification > 1106 Human Movement and Sports Science
Keywords ResPubID22097. skeletal muscle, buffer capacity, lactate transport, hydrogen ions, blood lactate, intense exercise, MCT, pH
Citations in Scopus 38 - View on Scopus
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