Not all J domains are created equal: implications for the specificity of Hsp40-Hsp70 interactions

Full text for this resource is not available from the Research Repository.

Hennessy, Fritha, Nicoll, Williams S, Zimmermann, Richard, Cheetham, Michael E and Blatch, Gregory ORCID: 0000-0003-0778-8577 (2005) Not all J domains are created equal: implications for the specificity of Hsp40-Hsp70 interactions. Protein Science, 14 (7). pp. 1697-1709. ISSN 0961-8368


Heat shock protein 40s (Hsp40s) and heat shock protein 70s (Hsp70s) form chaperone partnerships that are key components of cellular chaperone networks involved in facilitating the correct folding of a broad range of client proteins. While the Hsp40 family of proteins is highly diverse with multiple forms occurring in any particular cell or compartment, all its members are characterized by a J domain that directs their interaction with a partner Hsp70. Specific Hsp40–Hsp70 chaperone partnerships have been identified that are dedicated to the correct folding of distinct subsets of client proteins. The elucidation of the mechanism by which these specific Hsp40–Hsp70 partnerships are formed will greatly enhance our understanding of the way in which chaperone pathways are integrated into finely regulated protein folding networks. From in silico analyses, domain swapping and rational protein engineering experiments, evidence has accumulated that indicates that J domains contain key specificity determinants. This review will critically discuss the current understanding of the structural features of J domains that determine the specificity of interaction between Hsp40 proteins and their partner Hsp70s. We also propose a model in which the J domain is able to integrate specificity and chaperone activity.

Dimensions Badge

Altmetric Badge

Additional Information

Online ISSN: 1469-896X

Item type Article
DOI 10.1110/ps.051406805
Official URL
Subjects Historical > Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
Historical > FOR Classification > 0601 Biochemistry and Cell Biology
Historical > FOR Classification > 0604 Genetics
Keywords ResPubID22214. heat shock proteins, J domain, DnaJ, Hsp70s, Hsp40s, specificity determinants, DnaK
Citations in Scopus 237 - View on Scopus
Download/View statistics View download statistics for this item

Search Google Scholar

Repository staff login