Rational mutagenesis of a 40 kDa heat shock protein from Agrobacterium tumefaciens identifies amino acid residues critical to its in vivo function

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Hennessy, F, Boshoff, A and Blatch, Gregory L (2005) Rational mutagenesis of a 40 kDa heat shock protein from Agrobacterium tumefaciens identifies amino acid residues critical to its in vivo function. The International Journal of Biochemistry and Cell Biology, 37 (1). pp. 177-191. ISSN 1357-2725

Abstract

Prokaryotic DnaJ and DnaK, homologous to the eukaryotic 40 and 70 kDa heat shock proteins (Hsp40 and Hsp70) respectively, play an important role as molecular chaperones in assisted protein folding under both normal and stressed conditions. DnaJ-like proteins are defined by the presence of a 70 amino acid domain termed the J domain, similar to the initial 73 amino acids of the Escherichia coli protein DnaJ. The J domain comprises four α-helices and a loop region containing the invariant tripeptide of histidine, proline and aspartic acid (HPD motif). This motif and Helix II have been shown previously to be important for the interaction with partner Hsp70s. Conserved amino acid residues present in the J domain were identified, and substitutions of these residues were performed to examine their effect on the in vivo functioning of the J domain of Agrobacterium tumefaciens DnaJ. Three conserved, charged residues, and three conserved, hydrophobic residues, in addition to the HPD motif, were shown to be important for the correct functioning of A. tumefaciens DnaJ. These included Arg26 located on Helix II, Arg63 and Asp59 located on Helix IV, Tyr7 and Leu10 located on Helix I, and Leu57 located on Helix III. This study has identified charged and hydrophobic residues on all the structural elements of the J domain that were critical to the structure and function of DnaJ, and in particular shown that Helix IV may have an important role in the structure and function of DnaJs in general.

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Additional Information

Online ISSN: 1878-5875

Item type Article
URI https://vuir.vu.edu.au/id/eprint/8160
DOI https://doi.org/10.1016/j.biocel.2004.06.009
Official URL http://dx.doi.org/10.1016/j.biocel.2004.06.009
Subjects Historical > Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
Current > FOR Classification > 0601 Biochemistry and Cell Biology
Current > FOR Classification > 0604 Genetics
Keywords ResPubID22215. DnaJ, DnaK, J domain, Complementation assays, ATPase assays, Agrobacterium tumefaciens, heat shock proteins, 40 kDa
Citations in Scopus 29 - View on Scopus
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