An acute bout of high-intensity interval training increases the nuclear abundance of PGC-1α and activates mitochondrial biogenesis in human skeletal muscle

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Little, Jon, Safdar, Adeel, Bishop, David, Tarnopolsky, Mark and Gibala, Martin J (2011) An acute bout of high-intensity interval training increases the nuclear abundance of PGC-1α and activates mitochondrial biogenesis in human skeletal muscle. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology, 300 (6). R1303-R1310. ISSN 0363-6119 (print) 1522-1490 (online)

Abstract

Low-volume, high-intensity interval training (HIT) increases skeletal muscle mitochondrial capacity, yet little is known regarding potential mechanisms promoting this adaptive response. Our purpose was to examine molecular processes involved in mitochondrial biogenesis in human skeletal muscle in response to an acute bout of HIT. Eight healthy men performed 4 × 30-s bursts of all-out maximal intensity cycling interspersed with 4 min of rest. Muscle biopsy samples (vastus lateralis) were obtained immediately before and after exercise, and after 3 and 24 h of recovery. At rest, the majority of peroxisome proliferator-activated receptor γ coactivator (PGC)-1α, a master regulator of mitochondrial biogenesis, was detected in cytosolic fractions. Exercise activated p38 MAPK and AMPK in the cytosol. Nuclear PGC-1α protein increased 3 h into recovery from exercise, a time point that coincided with increased mRNA expression of mitochondrial genes. This was followed by an increase in mitochondrial protein content and enzyme activity after 24 h of recovery. These findings support the hypothesis that an acute bout of low-volume HIT activates mitochondrial biogenesis through a mechanism involving increased nuclear abundance of PGC-1α.

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Item type Article
URI https://vuir.vu.edu.au/id/eprint/9239
DOI https://doi.org/10.1152/ajpregu.00538.2010
Official URL http://ajpregu.physiology.org/content/300/6/R1303
Subjects Current > FOR Classification > 1106 Human Movement and Sports Science
Historical > SEO Classification > 970111 Expanding Knowledge in the Medical and Health Sciences
Historical > Faculty/School/Research Centre/Department > Institute of Sport, Exercise and Active Living (ISEAL)
Keywords ResPubID23884, exercise, mitochondria peroxisome, peroxisome proliferator-activated receptors, protein kinases
Citations in Scopus 185 - View on Scopus
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