A sensitive HPLC method for measuring bacterial proteolysis and proteinase activity in UHT milk
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Le, T X, Datta, Nivedita and Deeth, H. C (2006) A sensitive HPLC method for measuring bacterial proteolysis and proteinase activity in UHT milk. Food Research International, 39 (7). pp. 823-830. ISSN 0963-9969
Abstract
A sensitive quantitative reversed-phase HPLC method is described for measuring bacterial proteolysis and proteinase activity in UHT milk. The analysis is performed on a TCA filtrate of the milk. The optimum concentration of TCA was found to be 4%; at lower concentrations, non-precipitated protein blocked the HPLC while higher concentrations yielded lower amounts of peptides. The method showed greater sensitivity and reproducibility than a fluorescamine-based method. Quantification of the HPLC method was achieved by use of an external dipeptide standard or a standard proteinase.
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| Item type | Article |
| URI | https://vuir.vu.edu.au/id/eprint/3107 |
| DOI | 10.1016/j.foodres.2006.03.008 |
| Official URL | http://www.sciencedirect.com/science/article/pii/S... |
| Subjects | Historical > FOR Classification > 0305 Organic Chemistry Historical > Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences |
| Keywords | ResPubID17940, bacterial proteinase; HPLC, fluorescamine, proteinase activity, proteolysis |
| Citations in Scopus | 15 - View on Scopus |
| Download/View statistics | View download statistics for this item |
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