Optimization of cholesterol removal by probiotics in the presence of prebiotics by using a response surface method

1895 668 2200 6 23713 document 2200 (1895)RSM_292_cholesterol.pdf application/pdf 265327 2011-09-09 10:22:32 https://vuir.vu.edu.au/1895/1/%281895%29RSM_292_cholesterol.pdf 1895 1 application/pdf text en staffonly

(1895)RSM_292_cholesterol.pdf

http://eprints.org/relation/hasVolatileVersion https://vuir.vu.edu.au/id/document/10209 http://eprints.org/relation/hasVersion https://vuir.vu.edu.au/id/document/10209 http://eprints.org/relation/hasIndexCodesVersion https://vuir.vu.edu.au/id/document/10209 1 514cd4b5fc8f234c0543fb3886c7b032eb744e06 e07ee52a1b5fff2157174fc27309ae3bce9f6440 10209 3 1733002 document 10209 indexcodes.txt text/plain 39d90cc1f9bc46965f1e2069618c5f51 MD5 12530 2013-02-06 13:09:34 https://vuir.vu.edu.au/1895/2/indexcodes.txt 1895 2 2 text/plain other Generate index codes conversion from application/pdf to indexcodes en staffonly

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http://eprints.org/relation/isVersionOf https://vuir.vu.edu.au/id/document/2200 http://eprints.org/relation/isVolatileVersionOf https://vuir.vu.edu.au/id/document/2200 http://eprints.org/relation/issmallThumbnailVersionOf https://vuir.vu.edu.au/id/document/2200 archive 293 disk0/00/00/18/95 2009-07-03 04:13:06 2021-08-27 13:56:41 2010-10-04 04:21:46 article show Liong Min Tze Shah Nagendra P Shah, Negendra P Optimization of cholesterol removal by probiotics in the presence of prebiotics by using a response surface method pub 210000 tafeengineering carbohydrates metabolism, cholesterol metabolism, lactobacillus casei growth and development, models, biological, probiotics metabolism Lactobacillus casei ASCC 292 was grown in the presence of six prebiotics, namely, sorbitol, mannitol, maltodextrin, high-amylose maize, fructooligosaccharide (FOS), and inulin, in order to determine the combination of probiotic and prebiotics that would remove the highest level of cholesterol. A first-order model showed that the combination of L. casei ASCC 292, FOS, and maltodextrin was the most efficient for the removal of cholesterol, and the optimum experimental region was developed by using the steepest ascent. This led to the middle points of probiotic (1.70% [wt/vol]), FOS (4.80% [wt/vol]), and maltodextrin (6.80% [wt/vol]) for the development of a central composite design for optimization. Perturbation plot, response surface, and coefficient estimates showed that all three factors had significant quadratic effects on cholesterol removal, with FOS showing the most conspicuous quadratic change. A second-order polynomial regression model estimated that the optimum condition of the factors for cholesterol removal by L. casei ASCC 292 is 1.71% (wt/vol) probiotic, 4.95% (wt/vol) FOS, and 6.62% (wt/vol) maltodextrin. Validation experiments showed that the predicted optimum conditions were more efficient than the high and low levels of the factors and the center points. A response surface method proved reliable for developing the model, optimizing factors, and analyzing interaction effects. Analyses of growth, substrate utilization, growth yield, mean doubling time, and short-chain fatty acid (SCFA) production by the use of quadratic models indicated that cholesterol removal was growth associated. The concentration of L. casei ASCC 292 had the most significant quadratic effect on all responses studied, except for substrate utilization and SCFA production, which were significantly (P < 0.05) influenced by the interactions between the probiotic and both prebiotics, indicating that they were closely associated with the uptake of prebiotics. 2005 published American Society for Microbiology 10.1128/AEM.71.4.1745-1753.2005 restricted Applied and environmental microbiology 71 4 1745-1753 TRUE 0099-2240 http://aem.asm.org/cgi/content/abstract/71/4/1745 includes bibliographical references: p.1753 FALSE 61 2-s2.0-17444415023 2023-07-02 04:01:58