Optimization of cholesterol removal, growth and fermentation patterns of Lactobacillus acidophilus ATCC 4962 in the presence of mannitol, fructo-oligosaccharide and inulin: a response surface methodology approach

1896 685 2142 7 23768 document 2142 RSM_4962_cholesterol_JAM_rev1_(2).pdf application/pdf 311693 2011-09-09 10:22:32 https://vuir.vu.edu.au/1896/1/RSM_4962_cholesterol_JAM_rev1_%282%29.pdf 1896 1 application/pdf text en public

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RSM_4962_cholesterol_JAM_rev1_(2).pdf

http://eprints.org/relation/isVersionOf https://vuir.vu.edu.au/id/document/2142 http://eprints.org/relation/isVolatileVersionOf https://vuir.vu.edu.au/id/document/2142 http://eprints.org/relation/isCoversheetVersionOf https://vuir.vu.edu.au/id/document/2142 archive 293 disk0/00/00/18/96 2009-07-03 03:44:15 2021-08-27 13:56:47 2010-10-18 02:56:03 article show Liong Min Tze Shah Nagendra P Shah, Negendra P Optimization of cholesterol removal, growth and fermentation patterns of Lactobacillus acidophilus ATCC 4962 in the presence of mannitol, fructo-oligosaccharide and inulin: a response surface methodology approach pub 320000 tafeengineering lactic acid bacteria, bacteria, lactobacillaceae, applied microbiology, prebiotics, inoculum, methodology, inulin, oligosaccharide, mannitol, lactobacillus acidophilus, fermentation, growth, cholesterol, optimization Aims: To optimize cholesterol removal by Lactobacillus acidophilus ATCC 4962 in the presence of prebiotics, and study the growth and fermentation patterns of the prebiotics. Methods and Results: Lactobacillus acidophilus ATCC 4962 was screened in the presence of six prebiotics, namely sorbitol, mannitol, maltodextrin, hi-amylose maize, fructo-oligosaccharide (FOS) and inulin in order to determine the best combination for highest level of cholesterol removal. The first-order model showed that the combination of inoculum size, mannitol, FOS and inulin was best for removal of cholesterol. The second-order polynomial regression model estimated the optimum condition of the factors for cholesterol removal by L. acidophilus ATCC 4962 to be 2.64% w/v inoculum size, 4.13% w/v mannitol, 3.29% w/v FOS and 5.81% w/v inulin. Analyses of growth, mean doubling time and short-chain fatty acid (SCFA) production using quadratic models indicated that cholesterol removal and the production of SCFA were growth associated. Conclusions: Optimum cholesterol removal was obtained from the fermentation of L. acidophilus ATCC 4962 in the presence of mannitol, FOS and inulin. Cholesterol removal and the production of SCFA appeared to be growth associated and highly influenced by the prebiotics. Significance and Impact of the Study: Response surface methodology proved reliable in developing the model, optimizing factors and analysing interaction effects. The results provide better understanding on the interactions between probiotic and prebiotics for the removal of cholesterol. 2005 published Wiley-Blackwell Publishing Ltd 10.1111/j.1365-2672.2005.02544.x http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2005.02544.x/abstract 33021 2 2309754 dark_document 33021 Shah, N.pdf application/pdf 36bcbfba6ef7d357b9ccc8a15d911e0a MD5 41638 2018-09-20 07:28:51 1896 3 3 application/pdf text Permission form en staffonly

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other public Journal of Applied Microbiology 98 5 1115-1126 TRUE 1364-5072 Includes bibliographical references FALSE 42 2-s2.0-17644396977 2023-07-02 04:01:59