The purpose of this study was to identify the muscular activation profiles utilised by female athletes during a sport-specific landing task, and examine the effects of varying profiles on biomechanical anterior cruciate ligament (ACL) injury risk factors. Muscular activation profiles were identified from electromyography data using a combination of principal component and cluster analysis methods, with the neuromuscular and biomechanical characteristics of profiles compared. Various muscular activation characteristics contributed to the presence of lower limb biomechanical patterns consistent with ACL injury risk factors. Reduction of ACL injury risk may be achieved by targeting these muscular activation characteristics via neuromuscular training programs.