The cross-sector Guide has been developed from an 18 month intervention project funded by the Australian Government. The aim of this project was to work beyond sector silos and bring leaders from three male-dominated, cross-sector organisations together (sport, trades and surgery) to: gain insights into challenges women encounter when moving into or during their time as leaders; learn about effective global organisational practices to attract and retain women as leaders; implement an intervention to enhance practices in the workplace that support women in leadership positions; and discuss and address challenges faced during the intervention. The Guide presents pre- and post- program changes, good practices implemented and recommendations to enhance practices to the next level to encourage women as leaders.