This toolkit introduces the Regenerative City Living Lab (RCLL) applied research project and the toolkits that have been developed in association with this project and their purpose. The toolkits both report on the research undertaken as part of this project, but also provide learning resources in the areas of community-based research, human centred design, storytelling and environmental action. The RCLL project was led by Victoria University (VU) in 2021-2022 and in partnership with Social Enterprise STREAT. The project was funded by the Victorian State Government through the VU RISE (Recover, Innovate, Sustain and Evolve) initiative, which sought to conduct applied research in partnership with industry, community and government in the west of Melbourne to create solutions and deliver impact that will support our region to respond to the challenges of today’s world.