This study investigated the antimicrobial (AM) activity of starch-based films coated with linalool, carvacrol, or thymol against Staphylococcus aureus in vitro or inoculated on the surface of Cheddar cheese. In solid media using the agar diffusion method, the inhibitory effect of linalool, carvacrol, or thymol coated onto the films increased significantly (P≤ 0.05) with the increase in concentration of each AM agent. All the coated films effectively inhibited the growth of S. aureus on the surface of Cheddar cheese. The sensitivity of S. aureus to the AM agents tested in the concentration range of the study is in the order of thymol > carvacrol > linalool.