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Isolation and characterization of microsatellite markers in the musk duck (Biziura lobata: Aves), and their application to other waterfowl species

Guay, Patrick-Jean and Mulder, R (2005) Isolation and characterization of microsatellite markers in the musk duck (Biziura lobata: Aves), and their application to other waterfowl species. Molecular Ecology Notes, 5. pp. 249-252. ISSN 1471-8278

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Abstract

We isolated and characterized 11 novel microsatellite loci to study paternity in the Australian musk duck (Biziura lobata), using nonradioactive PCR-based techniques to screen GA and GAAA repeats enriched genomic DNA libraries. Nine of 11 loci showed no evidence of null alleles and were variable (mean HE = 0.825, mean number of alleles = 9). This set of nine loci is suitable for paternity assignment (exclusion probability for nine unlinked loci = 0.9999). We also demonstrated that many of these loci cross-amplify in various other waterfowl species.

Item Type: Article
Uncontrolled Keywords: ResPubID17196; Anatidae, Biziura lobata, microsatellite, musk ducks, parentage
Subjects: Faculty/School/Research Centre/Department > Institute for Sustainability and Innovation (ISI)
SEO Classification > 8398 Environmentally Sustainable Animal Production
Depositing User: VUIR
Date Deposited: 28 Mar 2012 05:03
Last Modified: 28 Mar 2012 05:03
URI: http://vuir.vu.edu.au/id/eprint/2761
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