Isolation and characterization of microsatellite markers in the musk duck (Biziura lobata: Aves), and their application to other waterfowl species
Guay, Patrick-Jean and Mulder, R (2005) Isolation and characterization of microsatellite markers in the musk duck (Biziura lobata: Aves), and their application to other waterfowl species. Molecular Ecology Notes, 5. pp. 249-252. ISSN 1471-8278Full text for this resource is not available from the Research Repository.
We isolated and characterized 11 novel microsatellite loci to study paternity in the Australian musk duck (Biziura lobata), using nonradioactive PCR-based techniques to screen GA and GAAA repeats enriched genomic DNA libraries. Nine of 11 loci showed no evidence of null alleles and were variable (mean HE = 0.825, mean number of alleles = 9). This set of nine loci is suitable for paternity assignment (exclusion probability for nine unlinked loci = 0.9999). We also demonstrated that many of these loci cross-amplify in various other waterfowl species.
|Uncontrolled Keywords:||ResPubID17196; Anatidae, Biziura lobata, microsatellite, musk ducks, parentage|
|Subjects:||Faculty/School/Research Centre/Department > Institute for Sustainability and Innovation (ISI)
SEO Classification > 8398 Environmentally Sustainable Animal Production
|Date Deposited:||28 Mar 2012 05:03|
|Last Modified:||28 Mar 2012 05:03|
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