Isolation and characterization of microsatellite markers in the musk duck (Biziura lobata: Aves), and their application to other waterfowl species

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Guay, Patrick-Jean and Mulder, R (2005) Isolation and characterization of microsatellite markers in the musk duck (Biziura lobata: Aves), and their application to other waterfowl species. Molecular Ecology Notes, 5. pp. 249-252. ISSN 1471-8278

Abstract

We isolated and characterized 11 novel microsatellite loci to study paternity in the Australian musk duck (Biziura lobata), using nonradioactive PCR-based techniques to screen GA and GAAA repeats enriched genomic DNA libraries. Nine of 11 loci showed no evidence of null alleles and were variable (mean HE = 0.825, mean number of alleles = 9). This set of nine loci is suitable for paternity assignment (exclusion probability for nine unlinked loci = 0.9999). We also demonstrated that many of these loci cross-amplify in various other waterfowl species.

Item type Article
URI https://vuir.vu.edu.au/id/eprint/2761
Official URL http://onlinelibrary.wiley.com/doi/10.1111/j.1471-...
Subjects Historical > Faculty/School/Research Centre/Department > Institute for Sustainability and Innovation (ISI)
Historical > SEO Classification > 8398 Environmentally Sustainable Animal Production
Keywords ResPubID17196; Anatidae, Biziura lobata, microsatellite, musk ducks, parentage
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