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Trehalose promotes the survival of Saccaromyces cerevisiae during lethal ethanol stress, but does not influence growth under sublethal ethanol stress

Bandara, Ajith and Fraser, Sarah and Chambers, Paul J and Stanley, Grant A (2009) Trehalose promotes the survival of Saccaromyces cerevisiae during lethal ethanol stress, but does not influence growth under sublethal ethanol stress. FEMS Yeast Research, 9 (8). pp. 1208-1216. ISSN 1557-1356

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Abstract

Trehalose is known to protect cells from various environmental assaults; however, its role in the ethanol tolerance of Saccharomyces cerevisiae remains controversial. Many previous studies report correlations between trehalose levels and ethanol tolerance across a variety of strains, yet variations in genetic background make it difficult to separate the impact of trehalose from other stress response factors. In the current study, investigations were conducted on the ethanol tolerance of S. cerevisiae BY4742 and BY4742 deletion strains, tsl1D and nth1D, across a range of ethanol concentrations. It was found that trehalose does play a role in ethanol tolerance at lethal ethanol concentrations, but not at sublethal ethanol concentrations; differences of 20–40% in the intracellular trehalose concentration did not provide any growth advantage for cells incubated in the presence of sublethal ethanol concentrations. It was speculated that the ethanol concentration-dependent nature of the trehalose effect supports a mechanism for trehalose in protecting cellular proteins from the damaging effects of ethanol.

Item Type: Article
Uncontrolled Keywords: ResPubID17691. trehalose, ethanol stress, Saccharomyces cerevisiae
Subjects: Faculty/School/Research Centre/Department > School of Engineering and Science
FOR Classification > 1003 Industrial Biotechnology
SEO Classification > 8505 Renewable Energy
Depositing User: VUIR
Date Deposited: 31 Aug 2011 06:15
Last Modified: 29 Jan 2015 00:55
URI: http://vuir.vu.edu.au/id/eprint/4134
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