Research Repository

The ethanol stress response and ethanol tolerance of Saccharomyces cerevisiae

Stanley, Dragana and Bandara, Ajith and Chambers, Paul J and Fraser, Sarah and Stanley, Grant A (2010) The ethanol stress response and ethanol tolerance of Saccharomyces cerevisiae. Journal of Applied Microbiology, 109 (1). pp. 13-24. ISSN 1364-5072

Full text for this resource is not available from the Research Repository.

Abstract

Saccharomyces cerevisiae is traditionally used for alcoholic beverage and bioethanol production; however, its performance during fermentation is compromised by the impact of ethanol accumulation on cell vitality. This article reviews studies into the molecular basis of the ethanol stress response and ethanol tolerance of S. cerevisiae; such knowledge can facilitate the development of genetic engineering strategies for improving cell performance during ethanol stress. Previous studies have used a variety of strains and conditions, which is problematic, because the impact of ethanol stress on gene expression is influenced by the environment. There is however some commonality in Gene Ontology categories affected by ethanol assault that suggests that the ethanol stress response of S. cerevisiae is compromised by constraints on energy production, leading to increased expression of genes associated with glycolysis and mitochondrial function, and decreased gene expression in energy-demanding growth-related processes. Studies using genome-wide screens suggest that the maintenance of vacuole function is important for ethanol tolerance, possibly because of the roles of this organelle in protein turnover and maintaining ion homoeostasis. Accumulation of Asr1 and Rat8 in the nucleus specifically during ethanol stress suggests S. cerevisiae has a specific response to ethanol stress although this supposition remains controversial.

Item Type: Article
Uncontrolled Keywords: ResPubID21199, biotechnology, fermentation, molecular genetic, stress response, yeasts
Subjects: Faculty/School/Research Centre/Department > School of Engineering and Science
FOR Classification > 1003 Industrial Biotechnology
SEO Classification > 8505 Renewable Energy
Depositing User: VUIR
Date Deposited: 21 May 2012 01:44
Last Modified: 29 Jan 2015 00:55
URI: http://vuir.vu.edu.au/id/eprint/7068
DOI: 10.1111/j.1365-2672.2009.04657.x
ePrint Statistics: View download statistics for this item
Citations in Scopus: 41 - View on Scopus

Repository staff only

View Item View Item

Search Google Scholar