The effects of prolonged exercise on Na+, K+ - ATPase and SR Ca2+ regulation in humans

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Leppik, James Alexander (2004) The effects of prolonged exercise on Na+, K+ - ATPase and SR Ca2+ regulation in humans. Research Master thesis, Victoria University of Technology.


Prolonged exhaustive submaximal exercise in humans induces marked metabolic changes, but little is known about effects on muscle Na+, K+ATPase activity and sarcoplasmic reticulum (SR) Ca2+ regulation. We therefore investigated whether these processes were impaired during cycling exercise at 74.3±1.2% v02 peak (mean±SEM) continued until fatigue, in eight healthy subjects (v02 peak 3.93±0.69 l.min-1). A vastus lateralis muscle biopsy was taken at rest, at 10 and 45 min of exercise and at fatigue. Muscle was analysed for in-vitro Na+,K+ ATPase activity (maximal K+ -stimulated 3-0-methylfluorescein phosphatase, 3-0-MFPase activity), Na+K+ATPase content (3[H] ouabain binding sites), SR Ca2+ release rate induced by 4 chloro-m-cresol and Ca2+ uptake rate.

Additional Information

Master of Applied Science

Item type Thesis (Research Master thesis)
Subjects Historical > FOR Classification > 0301 Analytical Chemistry
Historical > FOR Classification > 1106 Human Movement and Sports Science
Historical > Faculty/School/Research Centre/Department > School of Sport and Exercise Science
Keywords Sodium/potassium ATPase, Sarcoplasmic reticulum, Calcium, Metabolism, Exercise physiology
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