The effects of prolonged exercise on Na+, K+ - ATPase and SR Ca2+ regulation in humans
Download
Download
DownloadExport
Leppik, James Alexander (2004) The effects of prolonged exercise on Na+, K+ - ATPase and SR Ca2+ regulation in humans. Research Master thesis, Victoria University of Technology.
Abstract
Prolonged exhaustive submaximal exercise in humans induces marked metabolic changes, but little is known about effects on muscle Na+, K+ATPase activity and sarcoplasmic reticulum (SR) Ca2+ regulation. We therefore investigated whether these processes were impaired during cycling exercise at 74.3±1.2% v02 peak (mean±SEM) continued until fatigue, in eight healthy subjects (v02 peak 3.93±0.69 l.min-1). A vastus lateralis muscle biopsy was taken at rest, at 10 and 45 min of exercise and at fatigue. Muscle was analysed for in-vitro Na+,K+ ATPase activity (maximal K+ -stimulated 3-0-methylfluorescein phosphatase, 3-0-MFPase activity), Na+K+ATPase content (3[H] ouabain binding sites), SR Ca2+ release rate induced by 4 chloro-m-cresol and Ca2+ uptake rate.
| Additional Information | Master of Applied Science |
| Item type | Thesis (Research Master thesis) |
| URI | https://vuir.vu.edu.au/id/eprint/17887 |
| Subjects | Historical > FOR Classification > 0301 Analytical Chemistry Historical > FOR Classification > 1106 Human Movement and Sports Science Historical > Faculty/School/Research Centre/Department > School of Sport and Exercise Science |
| Keywords | Sodium/potassium ATPase, Sarcoplasmic reticulum, Calcium, Metabolism, Exercise physiology |
| Download/View statistics | View download statistics for this item |
CORE (COnnecting REpositories)