Transcriptional changes associated with ethanol tolerance in Saccharomyces cerevisiae

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Stanley, Dragana, Stanley, Grant A, Chambers, Paul J, Borneman, Anthony and Fraser, Sarah (2010) Transcriptional changes associated with ethanol tolerance in Saccharomyces cerevisiae. Applied Microbiology and Biotechnology, 88 (1). pp. 231-239. ISSN 0175-7598 (print) 1432-0614 (online)

Abstract

Saccharomyces spp. are widely used for ethanol production; however, fermentation productivity is negatively affected by the impact of ethanol accumulation on yeast metabolic rate and viability. This study used microarray and statistical two-way ANOVA analysis to compare and evaluate gene expression profiles of two previously generated ethanol-tolerant mutants, CM1 and SM1, with their parent, Saccharomyces cerevisiae W303-1A, in the presence and absence of ethanol stress. Although sharing the same parentage, the mutants were created differently: SM1 by adaptive evolution involving long-term exposure to ethanol stress and CM1 using chemical mutagenesis followed by adaptive evolution-based screening. Compared to the parent, differences in the expression levels of genes associated with a number of gene ontology categories in the mutants suggest that their improved ethanol stress response is a consequence of increased mitochondrial and NADH oxidation activities, stimulating glycolysis and other energy-yielding pathways. This leads to increased activity of energy-demanding processes associated with the production of proteins and plasma membrane components, which are necessary for acclimation to ethanol stress. It is suggested that a key function of the ethanol stress response is restoration of the NAD+/NADH redox balance, which increases glyceraldehyde-3-phosphate dehydrogenase activity, and higher glycolytic flux in the ethanol-stressed cell. Both mutants achieved this by a constitutive increase in carbon flux in the glycerol pathway as a means of increasing NADH oxidation.

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Item type Article
URI https://vuir.vu.edu.au/id/eprint/7067
DOI https://doi.org/10.1007/s00253-010-2760-7
Official URL http://link.springer.com/article/10.1007%2Fs00253-...
Subjects Historical > Faculty/School/Research Centre/Department > School of Engineering and Science
Historical > FOR Classification > 1003 Industrial Biotechnology
Historical > SEO Classification > 8505 Renewable Energy
Keywords ResPubID21198, Saccharomyces, ethanol, stress, gene expression, mutants
Citations in Scopus 39 - View on Scopus
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