Expression of Bacillus amyloliquefaciens amylase and Vibrio alginolyticus protease A fusion genes

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Zappe, H, Blatch, Gregory L and Woods, David R (1992) Expression of Bacillus amyloliquefaciens amylase and Vibrio alginolyticus protease A fusion genes. Journal of General Microbiology, 138 (2). pp. 319-327. ISSN 0022-1287

Abstract

Previously we reported [Deane, S. M., Maharaj, R., Robb, F. T. & Woods, D. R. (1987) Journal of General Microbiology 133,2295-23021 that the production of a Vibrio dginoiyticus SDS-resistant alkaline serine protease (Pro A) cloned in Escherichia coii was characterized by a 12 h delay between the synthesis of an inactive precursor and secretion of active Pro A. Replacement of the V. dginoiyticus promoter region by the a-amylase promoter region from Bacillus amyloliquefaciens resulted in the simultaneous synthesis and secretion of Pro A in E. coZi. The V. dginoiyticusproA gene cloned on a shuttle vector did not produce active Pro A in Bacillus subtiiis. Although Pro A has a typical Gram-positive signal sequence, it was not functional in B. subtiZis. Replacement of the Pro A signal sequence with the a-amylase signal sequence resulted in the production of active Pro A in B. subtiiis.

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Item type Article
URI https://vuir.vu.edu.au/id/eprint/8185
DOI https://doi.org/10.1099/00221287-138-2-319
Official URL http://mic.sgmjournals.org/content/138/2/319.short
Subjects Historical > Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
Historical > FOR Classification > 0604 Genetics
Keywords ResPubID22240. amylase, protease, Pro A, protease A, DNA, gene, fusion genes
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