Zappe, H, Blatch, Gregory ORCID: 0000-0003-0778-8577 and Woods, David R (1992) Expression of Bacillus amyloliquefaciens amylase and Vibrio alginolyticus protease A fusion genes. Journal of General Microbiology, 138 (2). pp. 319-327. ISSN 0022-1287

Abstract

Previously we reported [Deane, S. M., Maharaj, R., Robb, F. T. & Woods, D. R. (1987) Journal of General Microbiology 133,2295-23021 that the production of a Vibrio dginoiyticus SDS-resistant alkaline serine protease (Pro A) cloned in Escherichia coii was characterized by a 12 h delay between the synthesis of an inactive precursor and secretion of active Pro A. Replacement of the V. dginoiyticus promoter region by the a-amylase promoter region from Bacillus amyloliquefaciens resulted in the simultaneous synthesis and secretion of Pro A in E. coZi. The V. dginoiyticusproA gene cloned on a shuttle vector did not produce active Pro A in Bacillus subtiiis. Although Pro A has a typical Gram-positive signal sequence, it was not functional in B. subtiZis. Replacement of the Pro A signal sequence with the a-amylase signal sequence resulted in the production of active Pro A in B. subtiiis.

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