Induced mutations in cassava using somatic embryos and identification of mutant plants with altered starch yield and composition

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Joseph, Roy, Yeoh, Hock-Hin and Loh, Chiang-Shiong (2004) Induced mutations in cassava using somatic embryos and identification of mutant plants with altered starch yield and composition. Plant Cell Reports, 23 (1-2). pp. 91-98. ISSN 0721-7714

Abstract

A cyclic somatic embryogenic system was used to induce mutations in cassava variety PRC 60a in vitro. Globular-stage somatic embryos were selected as suitable experimental materials, and 50 Gy of -rays was determined to be the optimal dose for inducing mutations. During subsequent field trials, more than 50% of the regenerated mutant lines varied morphologically from wild-type plants. Consequently, we used this approach to induce genetic variability for obtaining novel cassava cultivars. Among the different mutant lines obtained, lines S14 and S15 showed large morphological variations. In 10-month-old S14 and S15 mutant lines, storage root yield was reduced 17-fold and 60-fold, respectively, compared to wild-type plants, while the storage roots of S15 mutant plants also exhibited an almost 50% decrease in starch content and a significant reduction (30%) in amylose content. These two features were observed throughout the different developmental stages of the storage roots in S15 plants.

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Item type Article
URI https://vuir.vu.edu.au/id/eprint/2606
DOI 10.1007/s00299-004-0798-7
Subjects Historical > Faculty/School/Research Centre/Department > School of Biomedical and Health Sciences
Keywords ResPubID18559, cassava, manihot esculenta, somatic embryos, starch biosynthesis
Citations in Scopus 44 - View on Scopus
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