Stanley, Dragana (2009) Generation and characterisation of ethanol-tolerant saccharomyces cerevisiae mutants. PhD thesis, Victoria University.

Abstract

Recent increases in the price of crude oil are providing impetus to develop alternative sources of energy, including biofuels. Ethanologenic microorganisms such as Saccharomyces cerevisiae are widely used by fermentation industries, and have been used for many years to produce BioEthanol. However the productivity of this microorganism is limited, in part because of its intolerance to the ethanol that it generates. As ethanol levels increase during fermentation, yeast metabolic rate and viability decrease which, in turn, compromises ethanol yield. Improving ethanol tolerance of yeast should, therefore, lead to increased fermentation productivity. To date, investigations on ethanol tolerance in yeast have mostly focused on analysis of defective (ethanol-sensitive) mutants. This study presents work describing the generation and characterisation of ethanol-tolerant mutants using an adaptive evolution approach on mutagenised and non-mutagenised.populations of S. cerevisiae W303-1A.

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